Oct 6: People with a range of rheumatic diseases have a new treatment delivery option after the FDA approved an intravenous formulation of Novartis IL-17A antagonist Cosentyx (secukinumab)
The figures on this page assume a 20 mg total blend vial: 3 mL of water gives ~6,667 mcg/mL , so 1 unit 66.7 mcg 250 mcg 4 units, 500 mcg 8 units
What a COA (Certificate of Analysis) Should Include Identity Confirmation Mass spectrometry confirming the 3,749.2 Da molecular weight (the full lipidated form, NOT the ~3,400 Da backbone) Amino acid sequence verification including the Lys-20 lipidation point Purity Testing HPLC purity look for 98%+ purity Chromatogram showing a single dominant peak with minimal truncated or unlipidated impurity peaks Contamination Testing Endotoxin levels (cagrilintide is injected subcutaneous) Residual solvent analysis (acetonitrile, TFA from SPMS cleavage) Heavy metals screening How to Verify a COA Is Legitimate Check the testing lab Janoshik Analytical, MZ Biolabs, and Colmaric Analyticals are the standard
Evaluation of N-glycosylation of secreted proteins N-glycosylation was evaluated by comparative WBs under basal conditions and after digestion with neuraminidase ((2 3, 6, 8, 9)) from Arthrobacter ureafaciens , Sigma-Aldrich), following the manufacturers instructions, and with N-glycosylase F (PNGase F, Sigma-Aldrich, Madrid, Spain)
Blocking it with 5-Amino-1MQ reactivated those cells and restored skeletal muscle regeneration in aged animal models
