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We initially shook the bottle trying to get the ice packs out, not realizing the vial was inside

Mano J, Ohno C, Domae Y, Asada K (2001) Chloroplastic ascorbate peroxidase is the primary target of methylviologen-induced photooxidative stress in spinach leaves: its relevance to monodehydroascorbate radical detected with in vivo ESR

We watch for common symptoms like swelling, joint pain, or fatigue

Selenium deficiency doesnt necessarily cause symptoms of deficiency, BUT there are some signs that you can look out for (which we will go over below)

The T2A sequence and FKBP-MIM I-BAR were inserted, followed by the Cas12f-Halo-FRB sequence of pKLV2-AsCas12fHKRA-Halo7-GSGS-FRB-guideRNA to express both Cas12f and MIM I-BAR by one plasmid in the cell