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Guglietta, S
The thermal cycling conditions composed of an initial denaturation step at 95 C for 1 min followed by 35 cycles of PCR using the following profile: 94 C for 30 s, 62 C for 30 s, and 72 C for 30 s using Bio-Rad CFX connect
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The first time the dog showed upthey're afraid of what's behind the fencehe got totally freaked out." But though her boy was "not quite of the right age," she continued, "kids who were maybe 5, 6, 7, 8, 9, 10 really connected with that film
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