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The group exposed to cigarette smoke had the smallest and lightest testes compared to those exposed to e-cigarette vapor and the control group
tabacum germplasms
Comparative analysis of the activities of recombinant TEV, ACP-TEV and TEV-R9 For comparative analysis of the activities of recombinant TEV and its ACP-TEV fusion protein, the substrate MBP-BCCP was mixed with TEV and ACP-TEV at a molar ratio of 10:1, and incubated at 25 C in standard reaction buffer (50 mM TRISHCl, 0.5 mM EDTA, 1 mM DDT, pH 8.0)
Pupae were sorted into 200 mL plastic cups and transferred to BugDorm-1 Insect Rearing Cages (30 30 30 cm, Bug dorm Company, Taichung, Taiwan)