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Unlike BSA and transferrin (Tfn), there was little uptake of AF594-CtxB in air exposed SAEC, however, there was a significant increase in the number of cells taking up CtxB after smoke exposure (Fig 4A and 4B)

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Although we did not fully characterize the composition of 3R4F reference cigarette smoke, we have estimated that there are 10-fold fewer chemical constituents present in THS aerosol and can confirm that these 529 compounds are also part of the 6,000+ compounds known to be present in cigarette smoke

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