In this study, we performed two of the above-mentioned protocols
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Once the tobacco can resist black shank, you have to back cross it with the original seed again to achieve the flavor characteristics. Seems like farming 101, but its a long process of selection that took four years to complete
The TCA cycle of in-vitro tobacco plants was impaired (flux reduction by 77%), with an increase of malate production from fumarate (2.9-fold) and from oxaloacetate (by 68%)