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Ways to Improve Effectiveness: Add a cardboard shroud No cost improvement Use thicker filters 4" rather than 1" MERV 13 filters Use multiple filters 2-5 filter design Key Reminders Only use certified fans with UL or FTL markings 2012 model or newer Keep extra filters on hand Replace filters when dirty

Both the frequency and amount should be reduced

Dimensionality reduction, clustering, and visualization Before clustering and visualizing each of the two scRNA-seq datasets (in vitro and in vivo), we reduced the dimensionality of variation in a way that accounted for batch-based shifts in expression among donors

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In cases of non-normal distributions, non-parametric alternatives (Mann-Whitney U test or Kruskal-Wallis test) were applied