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The sample separation was achieved on an Acclaim 120 C18 (2.1 mm 50 mm, 3 m, Thermo Scientific, Sunnyvale, CA) column with a flow rate of 0.3 mL/min and a column temperature of 30 C

Although most young people today acknowledge that smoking can be harmful, many underestimate the dangers and potential long-term effects of smoking

This challenges the effectiveness of current regulations and suggests the need for further and more consistent restrictions to protect young people from tobacco consumption [36]

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Surprisingly, second-hand smoke also affects sleep