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Images were analyzed using FiJi ImageJ software to calculate the percentage of CD31 positive areas

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I hope the results of this study can reach at least some smokers of Natural American Spirit cigarettes and help in correcting the misperception that these cigarettes are somehow healthier than other brands. This study was supported by the National Cancer Institute of the National Institutes of Health and the Center for Tobacco Products of the Food and Drug Administration, and by a Continuing Umbrella of Research Experiences supplement from the National Cancer Institute

We therefore performed a sensitivity analysis reducing F AA , F TA and the calculated RR of drinking ten units of alcohol, or smoking ten cigarettes per week, by a factor of 50% for cancers in which both alcohol and tobacco contribute (Additional file 1: Table S6)