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I asked a question one time like, "Why do you toss the filter?" It's like the only part of the cigarette that's saving you, but we toss it at the end
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As is expected for any model, cell culture experiments inherently have factors that need to be considered for reproducibility, such as choosing the most appropriate cell type/line, passage of the cells, confluency of cells during treatment, serum deprivation, treatment dose, duration, and handling techniques
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