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As a general rule, heavy smokers do well starting at the maximum nicotine strength available for retail - a 2.0% or 20mg/ml e-liquid, where light smokers may find starting at a lower strength of 1.8% or 1.5% to be satisfactory

The mixture was stirred for 4 h at room temperature and the condensation reaction was stopped by adding 1 mL of acetate buffer (4M, pH 4.75) to each reaction mixture