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qPCR was performed using the SmartGene Sybr Green Q-PCR Master Mix with Low Rox (SMART GENE) according to the manufacturers instructions twice to determine the expression of IL-1, IL-6, TNF-, VEGF, and HIF-1
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The study used a mixed effects zero-inflated discrete Weibull (ZIDW) regression model with a Bayesian approach, which is more suitable for longitudinal count data that are zero-inflated and skewed (contains outliers)
And boy, his response was wild: 40, 45
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