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[35] [36] By the mid-17th century, most major civilizations had been introduced to tobacco smoking and, in many cases, had already assimilated it into the native culture, despite some continued attempts on the part of rulers to eliminate the practice with penalties or fines

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Nicotine exposure-induced methylation changes in sperm DNA are partially reversible Testicular metabolomic analysis demonstrated an elevation in methyl donors in NIC mice, indicative of DNA methylation modifications

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Speech problems: Changes to the tongue and lips after surgery can affect speech