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Among 7-mg ISO tar smokers, slightly lower (5%) MLE to nicotine was found for the control product compared as with the split-tipping product ( P = 0.039)

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P-values for group comparisons derived from independent samples t-tests or Mann-Whitney U tests as appropriate

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Immunblots were performed from cell lysate to analyze the expression of different cell cycle and apoptotic markers, such as, p53, p21, Bax, Bcl-2 and from serum to measure the level of different pro-inflammatory cytokines, such as, IL-6, TNF-, iNOS, Cox-2