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The mobile phase consisted of 10 mM ammonium acetate in water (solvent A) and a 1:1 mixture of acetonitrile and isopropanol (solvent B), delivered at a flow rate of 0.30 mL/min

Based on their basic fundamental structures and functional substituents, the twelve flavonoids were classified as follows: one flavonol (kaempferol rutinoside), three flavanones (naringin, hesperidin, and neohesperidin), seven flavones (sinensetin, nobiletin, ternatin, 3-methoxynobiletin, tangeretin, 5-demethylnobiletin, and 5-hydroxy-7,8,3,4-tetramethoxyflavone), and one flavonone (citromitin)

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