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After 16 weeks, all animals were challenged by intratracheal instillation of nontypeable Haemophilus influenzae (NTHI), an opportunistic gram-negative bacterium responsible for respiratory tract infections and exacerbations in COPD patients [23], and euthanized 0, 4, and 12 h later

PMID 26520792

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which reported concentrations obtained by chromatography/mass spectrometry methods, positive results were found under repeated exposure or exposure conditions so extreme that the study subjects were offered goggles to wear in order to prevent eye and mucous membrane irritation due to the test area being visibly saturated with marijuana smoke